Revista de Microbiologia
Print version ISSN 0001-3714
CORREIA, Marcos José; PEREIRA JUNIOR, José Antônio de Sousa; SANTOS, Jefferson Cunha dos and CAVALCANTI, Maria Auxiliadora de Queiroz. Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes. Rev. Microbiol. [online]. 1998, vol.29, n.4, pp.286-288. ISSN 0001-3714. http://dx.doi.org/10.1590/S0001-37141998000400010.
A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).
Keywords : Avicel; cellulase; Remazol Blue; Lentinus edodes; Pleurotus ostreatus.