Revista de Microbiologia
versão impressa ISSN 0001-3714
SILVA, Claudio Henrique Cerri e; PULS, Jurgen; SOUSA, Marcelo Valle de e FERREIRA FILHO, Edivaldo Ximenes. Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius. Rev. Microbiol. [online]. 1999, vol.30, n.2, pp.114-119. ISSN 0001-3714. http://dx.doi.org/10.1590/S0001-37141999000200005.
A xylan-degrading enzyme (xylanase II) was purified to apparent homogeneity from solid-state cultures of Aspergillus fumigatus Fresenius. The molecular weight of xylanase II was found to be 19 and 8.5 kDa, as estimated by SDS-PAGE and gel filtration on FPLC, respectively. The purified enzyme was most active at 55 °C and pH 5.5. It was specific to xylan. The apparent Km and Vmax values on soluble and insoluble xylans from oat spelt and birchwood showed that xylanase II was most active on soluble birchwood xylan. Studies on hydrolysis products of various xylans and xylooligomers by xylanase II on HPLC showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase activity.
Palavras-chave : xylan; xylanase; Aspergillus fumigatus.