Revista de Microbiologia
versão impressa ISSN 0001-3714
SOARES, Márcia M.C.N.; SILVA, Roberto da e GOMES, Eleni. Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by Bacillus sp. Rev. Microbiol. [online]. 1999, vol.30, n.4, pp. 299-303. ISSN 0001-3714. http://dx.doi.org/10.1590/S0001-37141999000400002.
One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30% presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturonase production indicated that five strains of Bacillus sp produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0 - 7.0, optimum temperatures between 45oC and 55ºC, stability at temperatures above 40ºC and in neutral and alkaline pH, were determined.
Palavras-chave : Bacillus sp; polygalacturonase; pectinolytic activity.