Revista de Microbiologia
versão impressa ISSN 0001-3714
MAIA, Maria de Mascena Diniz et al. Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1. Rev. Microbiol. [online]. 1999, vol.30, n.4, pp. 304-309. ISSN 0001-3714. http://dx.doi.org/10.1590/S0001-37141999000400003.
A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25ºC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30ºC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50ºC. Lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.
Palavras-chave : Enzyme kinetics; ester hydrolysis; extracellular lipase; Fusarium solani.