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Anais da Academia Brasileira de Ciências
Print version ISSN 0001-3765
LIMA, Dirlene P. et al. Probiotics prevent growth deficit of colon wall strata of malnourished rats post-lactation. An. Acad. Bras. Ciênc. [online]. 2012, vol.84, n.3, pp.727-736. Epub July 17, 2012. ISSN 0001-3765. http://dx.doi.org/10.1590/S0001-37652012005000043.
The objective of this study was to analyze morphometrically the colon wall strata of malnourished rats supplemented with probiotics. Sixteen recently weaned Wistar rats (Rattus norvegicus) were distributed into four groups: animals that received commercial chow (G1, n = 4); animals that received the same feed as G1 and were supplemented with probiotics (G2, n = 4); animals that received chow with 4% of proteins (G3, n = 4); animals that received the same feed as G3 and were supplemented with probiotics (G4, n = 4). After 12 weeks, the proximal colon was collected and submitted to histological processing. Three-µm cuts were stained with H.E., Periodic Acid Schifff (P.A.S.) + diasthasis solution and Alcian Blue (A.B.) pH 2.5 and pH 1.0. The morphometric analysis of the intestinal wall showed that the supplementation with ABT-4 probiotic culture prevents the growth deficit of colon wall strata that normally occurs in malnourished rats right after lactation. Besides, no alteration was observed in the proportion of the number of globet cells in relation to the number of enterocytes in malnourished rats, regardless of the supplementation with probiotics.
Keywords : histology; large intestine; malnourishment; morphometry; probiotics.