Evaluation of analgesic and anti-inflammatory activities of <i>Hydrocotyle umbellata</i> L., Araliaceae (acariçoba) in mice

The Hydrocotyle umbellata L. is a specimen of the Araliaceae family popularly known as acariçoba. Its indications in folk medicine include treatment of skin ulcers, and rheumatism. The aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of the ethanolic extract from acariçoba's underground parts (EEA). EEA reduced the nociceptive response of the animals as evaluated in the acetic acid-induced writhing test and in both phases of formalin test. EEA also presented a supraspinal analgesic activity by increasing the pain latency in the hot plate test. Moreover, EEA reduced the leukocytes migration and plasma extravasation to pleural cavity in the carrageenan-induced pleurisy, besides reducing the edema induced by carrageenan until the second hour and also the edema induced by dextran. In conclusion our results showed that EEA of H. umbellata L. presents analgesic and anti-inflammatory activities, and that a blockade of activity or reduction in the release of different mediators, such as histamine and serotonin, could be involved in these pharmacologic effects.

Acariçoba; anti-inflammatory; antinociceptive; Biomedical Sciences; Hydrocotyle umbellata L

Authorship

IZIARA F. FLORENTINO

Laboratório de Farmacologia de Produtos Naturais, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970, Goiânia, GO, BrasilUniversidade Federal de GoiásBrasilGoiânia, GO, BrasilLaboratório de Farmacologia de Produtos Naturais, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970, Goiânia, GO, Brasil

MARCUS VINÍCIUS M. NASCIMENTO

PABLINNY M. GALDINO

ADRIANE F. DE BRITO

FABIO F. DA ROCHA

Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica, RJ, BrasilUniversidade Federal Rural do Rio de JaneiroBrasilSeropédica, RJ, BrasilDepartamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica, RJ, Brasil

CARLOS R. TONUSSI

Departamento de Farmacologia, Universidade Federal de Santa Catarina, 88049-900, Florianópolis, SC, BrasilUniversidade Federal de Santa CatarinaBrasilFlorianópolis, SC, BrasilDepartamento de Farmacologia, Universidade Federal de Santa Catarina, 88049-900, Florianópolis, SC, Brasil

THEREZA CHRISTINA M. DE LIMA

JOSÉ R. DE PAULA

Laboratório de Pesquisa de Produtos Naturais, Faculdade de Farmácia, Universidade Federal de Goiás, 74605-220, Goiânia, GO, BrasilUniversidade Federal de GoiásBrasilGoiânia, GO, BrasilLaboratório de Pesquisa de Produtos Naturais, Faculdade de Farmácia, Universidade Federal de Goiás, 74605-220, Goiânia, GO, Brasil

ELSON A. COSTA

Correspondence to: Elson Alves Costa E-mail: xico@icb.ufg.br

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (8)

Thumbnail

Figure 1
Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.) in the number of acetic acid-induced writhes in mice. Indomethacin (10 mg/kg p.o.) was used as positive control. Vertical bars represent mean ± SEM of cumulated writhings in 30 min for each experimental group. ^*p < 0.05; ^***p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Thumbnail

Figure 2
Effect of the ethanolic extract from acariçoba underground parts (EEA) (1,000 mg/kg p.o.), indomethacin (10 mg/kg p.o.) and morphine (10 mg/kg s.c.) on the licking time of Formalin-induced pain, in mice, in the first phase (0-5 min) and the second phase (15-30 min). Vertical bars represent mean ± SEM of reaction time pain, in seconds. ^*** p < 0.001 vs first phase control; ## p < 0.01 and ### p < 0.001 vs second phase control. According ANOVA followed by Student-Newman-Keuls' test.

Thumbnail

Figure 3
Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.) or morphine (10 mg/kg s.c.) on the nociceptive response of mice in the hot-plate test. Values are expressed as mean ± SEM of the latency for the nociceptive behavior. ^* p < 0.05; ^*** p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Thumbnail

Figure 4
Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.), in carrageenan-induced pleurisy in mice. Dexamethasone (2.0 mg/kg p.o.) was used as positive control. The bars represent the means ± SEM of number of migrated leukocytes /mL (× 10⁶) migrated to pleural cavity after carrageenan injection relative to control group. ^*** p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Thumbnail

Figure 5
Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.), in carrageenan-induced pleurisy in mice. Dexamethasone (2.0 mg/kg p.o.) was used as positive control. The bars represent the means ± SEM of Evan's blue concentration (µg/mL) in the pleural exudate after carrageenan injection. ^** p < 0.01 and ^*** p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Thumbnail

Figure 6
Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.), in croton oil-induced ear edema in mice. Dexamethasone (2.0 mg/kg p.o.) was used as positive control. Vertical bars represent mean ± SEM of the difference between left and right ear plugs, in milligrams. ^* p < 0.05; ^** p < 0.01; ^*** p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Thumbnail

Figure 7
Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.) or indomethacin (10 mg/kg p.o.) on the carrageenan-induced paw edema. The edema was measured 1, 2, 3 and 4 h after the injection of the phogistic agent. Vertical bars represent mean ± SEM of the difference between the volumes of the paws. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Thumbnail

Figure 8
Effect of the ethanolic extract from acariçoba underground parts (EEA) (1,000 g/kg p.o.) or cyproheptadine (5.0 mg/kg p.o.) on the dextran-induced paw edema. The edema was measured 0, 30, 60, 90 and 120 min after the injection of dextran. Vertical bars represent mean ± SEM of the difference between the volumes of the paws. ^* p < 0.05 and ^** p < 0.01. According ANOVA followed by Student-Newman-Keuls' test.

Figure 1 Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.) in the number of acetic acid-induced writhes in mice. Indomethacin (10 mg/kg p.o.) was used as positive control. Vertical bars represent mean ± SEM of cumulated writhings in 30 min for each experimental group. ^*p < 0.05; ^***p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Figure 2 Effect of the ethanolic extract from acariçoba underground parts (EEA) (1,000 mg/kg p.o.), indomethacin (10 mg/kg p.o.) and morphine (10 mg/kg s.c.) on the licking time of Formalin-induced pain, in mice, in the first phase (0-5 min) and the second phase (15-30 min). Vertical bars represent mean ± SEM of reaction time pain, in seconds. ^*** p < 0.001 vs first phase control; ## p < 0.01 and ### p < 0.001 vs second phase control. According ANOVA followed by Student-Newman-Keuls' test.

Figure 3 Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.) or morphine (10 mg/kg s.c.) on the nociceptive response of mice in the hot-plate test. Values are expressed as mean ± SEM of the latency for the nociceptive behavior. ^* p < 0.05; ^*** p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Figure 4 Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.), in carrageenan-induced pleurisy in mice. Dexamethasone (2.0 mg/kg p.o.) was used as positive control. The bars represent the means ± SEM of number of migrated leukocytes /mL (× 10⁶) migrated to pleural cavity after carrageenan injection relative to control group. ^*** p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Figure 5 Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.), in carrageenan-induced pleurisy in mice. Dexamethasone (2.0 mg/kg p.o.) was used as positive control. The bars represent the means ± SEM of Evan's blue concentration (µg/mL) in the pleural exudate after carrageenan injection. ^ p < 0.01 and ^* p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Figure 6 Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.), in croton oil-induced ear edema in mice. Dexamethasone (2.0 mg/kg p.o.) was used as positive control. Vertical bars represent mean ± SEM of the difference between left and right ear plugs, in milligrams. ^* p < 0.05; ^ p < 0.01; ^* p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Figure 7 Effect of the ethanolic extract from acariçoba underground parts (EEA) (250, 500 and 1,000 mg/kg p.o.) or indomethacin (10 mg/kg p.o.) on the carrageenan-induced paw edema. The edema was measured 1, 2, 3 and 4 h after the injection of the phogistic agent. Vertical bars represent mean ± SEM of the difference between the volumes of the paws. ^p < 0.05; ^p < 0.01; ^p < 0.001. According ANOVA followed by Student-Newman-Keuls' test.

Figure 8 Effect of the ethanolic extract from acariçoba underground parts (EEA) (1,000 g/kg p.o.) or cyproheptadine (5.0 mg/kg p.o.) on the dextran-induced paw edema. The edema was measured 0, 30, 60, 90 and 120 min after the injection of dextran. Vertical bars represent mean ± SEM of the difference between the volumes of the paws. ^* p < 0.05 and ^** p < 0.01. According ANOVA followed by Student-Newman-Keuls' test.

How to cite

copy

Academia Brasileira de Ciências Rua Anfilófio de Carvalho, 29, 3º andar, 20030-060 Rio de Janeiro RJ Brasil, Tel: +55 21 3907-8100 - Rio de Janeiro - RJ - Brazil
E-mail: aabc@abc.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Evaluation of analgesic and anti-inflammatory activities of <i>Hydrocotyle umbellata</i> L., Araliaceae (acariçoba) in mice

PlumX

Mensagem

[1] Correspondence to: Elson Alves Costa E-mail: xico@icb.ufg.br