SciELO - Scientific Electronic Library Online

vol.86 issue3Hepatic steatosis associated with microsporidiosis in teleost fishes from Marajó Island, BrazilThe effect of seasonality on the structure of rotifers in a black-water shallow lake in Central Amazonia author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Anais da Academia Brasileira de Ciências

Print version ISSN 0001-3765


REGO, MOACYR J.B.M. et al. Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification. An. Acad. Bras. Ciênc. [online]. 2014, vol.86, n.3, pp.1351-1358.  Epub Aug 15, 2014. ISSN 0001-3765.

The present work aimed to magnetize Parkia pendula seeds gum and use it as a matrix for Concanavalin A covalent immobilization. This composite was applied in affinity purification of glycoconjugates. Parkia pendula seeds were hydrated and the gum provenient from the supernatant was precipitated and washed with ethanol and dried. The gum was magnetized in co-precipitation using solutions of Fe+2 and Fe+3. Matrix activation was accomplished with NaIO4. Magnetized Parkia pendula seeds gum with covalently immobilized Concanavalin A was used as an affinity matrix for the recognition of bovine serum fetuin glycoprotein. Fetuin elution was carried out with a solution of glucose (300mM) and evaluated through SDS-PAGE. The efficiency of lectin immobilization and fetuin purification were 63% and 14%, respectively. These results indicate that the composite produced is a promising magnetic polysaccharide matrix for lectins immobilization. Thus, such system can be applied for affinity purification allowing an easy recovery by magnetic field.

Keywords : immobilization; magnetization; Parkia pendula seed gum; Concanavalin A.

        · abstract in Portuguese     · text in English     · English ( pdf )