Sublethal effect of concentrations of Metarhizium anisopliae (metsch.) sorok on the larval stage and immunologic characteristics of Diatraea flavipennella (box) (lepidoptera: crambidae)

This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 10³, 10⁴, 10⁵ conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 10³, 10⁵, 10⁷ conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 10⁷ spores / mL (24), while the activity of phenoloxidase was similar among treatments in 24 and higher concentration 10⁷ spores / mL (60h). In biochemical profile of hemocytes was a change in carbohydrates, lipids and proteins in response to the fungus. The results indicate that the fungus M. anisopliae can be used in the Integrated Management of D. flavipennella by presenting pathogenicity and interfere with their development even when exposed to small concentrations.

Entomopathogen; hemocytes; histochemistry; nitric oxide; phenoloxidases; sugarcane borer