SciELO - Scientific Electronic Library Online

 
vol.87 issue1Novelties in secretory structures and anatomy of Rhynchosia(Fabaceae)Three new species of Cladonia (Cladoniaceae, Ascomycota) from Southern Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Anais da Academia Brasileira de Ciências

Print version ISSN 0001-3765

Abstract

SUTILI, FERNANDO J. et al. Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonassp. An. Acad. Bras. Ciênc. [online]. 2015, vol.87, n.1, pp.95-100.  Epub Mar 13, 2015. ISSN 0001-3765.  https://doi.org/10.1590/0001-3765201520130442.

In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonassp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.

Keywords : antibacterial activity; essential oils; fish; gentamicin.

        · abstract in Portuguese     · text in English     · English ( pdf )