SciELO - Scientific Electronic Library Online

vol.87 issue2Comments on Triassic pterosaurs with discussion about ontogeny and description of new taxaGrowth and reproduction of the mangrove crab Goniopsis cruentata (Latreille, 1803) (Crustacea: Decapoda: Grapsidae) in southeastern Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Anais da Academia Brasileira de Ciências

Print version ISSN 0001-3765On-line version ISSN 1678-2690


MONDINO, Eduardo A. et al. Extracting DNA of nematodes communities from Argentine Pampas agricultural soils. An. Acad. Bras. Ciênc. [online]. 2015, vol.87, n.2, pp.691-697. ISSN 0001-3765.

We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.

Keywords : Agroecosystems; nematodes; nucleic acid extraction; CTAB.

        · abstract in Portuguese     · text in English     · English ( pdf )