Anesthesia of <i>Epinephelus marginatus</i> with essential oil of <i>Aloysia polystachya</i>: an approach on blood parameters

FOGLIARINI, CARINE O.; GARLET, QUELEN I.; PARODI, THAYLISE V.; BECKER, ALEXSSANDRO G.; GARCIA, LUCIANO O.; HEINZMANN, BERTA M.; PEREIRA, ANA MARIA S.; BALDISSEROTTO, BERNARDO

doi:10.1590/0001-3765201720160457

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Biological Sciences • An. Acad. Bras. Ciênc. 89 (1 suppl) • 2017 • https://doi.org/10.1590/0001-3765201720160457 copy

Anesthesia of Epinephelus marginatus with essential oil of Aloysia polystachya: an approach on blood parameters

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

This study investigated the anesthetic potential of the essential oil (EO) of Aloysia polystachya in juveniles of dusky grouper (Epinephelus marginatus). Fish were exposed to different concentrations of EO of A. polystachya to evaluate time of induction and recovery from anesthesia. In the second experiment, fish were divided into four groups: control, ethanol and 50 or 300 µL L⁻¹ EO of A. polystachya, and each group was submitted to induction for 3.5 min and recovery for 5 or 10 min. The blood gases and glucose levels showed alterations as a function of the recovery times, but Na⁺ and K⁺ levels did not show any alteration. In conclusion, the EO from leaves of A. polystachya is an effective anesthetic for dusky grouper, because anesthesia was reached within the recommended time at EO concentrations of 300 and 400 µL L⁻¹. However, most evaluated blood parameters showed compensatory responses due to EO exposure.

Key words:
anesthetic efficacy; blood gases; glucose; hemoglobin; plasma ion levels

EO concentration (µL L⁻¹)	Sedation	Anesthesia	Recovery
50	429.3 ± 12.2	-	-
75	285.8 ± 15.3	-	-
100	210.0 ± 3.5	1555.7 ± 46.7	565.0 ± 9.3^a
200	96.3 ± 3.4	313.4 ± 16.8	421.4 ± 17.3^b
300	50.7 ± 0.7	163.7 ± 16.5	480.8 ± 8.7^b
400	46.5 ± 1.6	90.2 ± 3.2	330.5 ± 13.0^c
Equations	y = 684.4 e^(−0.01x) r² = 0.9529	y = 6424.5 e^(−0.01x) r² = 0.9759	-

	pH	PvO₂ (mm Hg)	PvCO₂ (mm Hg)	HCO₃ ⁻ (mmoL L⁻¹)
Control
Exposure	7.40 ± 0.06^a	20.03 ± 0.95ª	14.25 ± 0.80ª	9.89 ± 0.54^a
Recovery 5 min	7.37 ± 0.04^a	37.66 ± 3.40^b	8.79 ± 0.81^b	5.98 ± 0.32^b
Recovery 10 min	7.39 ± 0.07^a	35.05 ± 3.63^b	8.79 ± 0.90^b	5.98 ± 0.42^b
Ethanol
Exposure	7.32 ± 0.08^a	25.74 ± 4.34ª	15.12 ± 1.93^a	8.38 ± 0.60ª
Recovery 5 min	7.42 ± 0.06^a	40.81 ± 3.68^b	8.29 ± 0.72^b	5.36 ± 0.24^c
Recovery 10 min	7.38 ± 0.03^a	25.05 ± 4.28ª	11.24 ± 0.78^a	6.55 ± 0.07^b
EO 50 µL L⁻¹
Exposure	7.49 ± 0.03^a	60.97 ± 3.39ª*^#	11.89 ± 0.83ª	9.18 ± 0.66ª
Recovery 5 min	7.46 ± 0.05^a	42.79 ± 5.90^b	8.74 ± 1.05ª	5.25 ± 0.43^b
Recovery 10 min	7.40 ± 0.05^a	36.08 ± 3.70^b	9.20 ± 0.68ª	5.72 ± 0.32^b
EO 300 µL L⁻¹
Exposure	7.28 ± 0.06^a	37.48 ± 3.16ª*	19.53 ± 0.78^a*	8.93 ± 0.81ª
Recovery 5 min	7.19 ± 0.05^a	61.20 ± 4.69^b*^#	11.85 ± 0.93^b*^#	4.65 ± 0.46^c
Recovery 10 min	7.46 ± 0.07^a	44.24 ± 0.95^c*^#	8.30 ± 0.3^6c#	6.58 ± 0.48^b

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[1] Correspondence to: Bernardo Baldisserotto E-mail: bbaldisserotto@hotmail.com

Peak	RT	Compound	RI experimental	RI literature	Source	%
1	10.23	α-Pinene	931	937	N	0.75
2	11.94	β-Pinene	974	975	N	0.45
3	12.65	β-Myrcene	991	990	N	1.57
4	14.05	α-Limonene	1027	1028	N	33.68
5	14.52	β-E-Ocimene	1039	1038	N	0.40
6	14.91	β-Z-Ocimene	1049	1051	N	0.21
7	16.93	β-Linalool	1100	1098	N	0.93
8	17.69	E-p-Mentha-2,8-dienol	1121	1118	N	0.19
9	18.16	Limonene epoxide	1134	1136	N	0.27
10	20.28	α-Terpineol	1191	1190	N	0.40
11	20.43	1,6-Dihydrocarveol	1195	1195	N	0.29
12	20.50	Dihydrocarvone	1197	1199	N	0.29
13	21.70	E-Carveol	1231	1233	N	0.24
14	22.16	Carvone	1244	1242	N	58.76
15	23.22	Perillal	1275	1274	N/A	0.17
16	23.86	Thymol	1293	1292	N	0.16
17	27.99	β-Caryophyllene	1420	1419	N	0.80
18	29.05	α-Caryophyllene	1454	1455	N	0.15
19	30.74	β-Bisabolene	1509	1509	N	0.29
Total identified:				100.00