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Anais da Academia Brasileira de Ciências

Print version ISSN 0001-3765On-line version ISSN 1678-2690

Abstract

LONDE, LUCIANA C.N.; VENDRAME, WAGNER A.; SANAEI, MASSY  and  OLIVEIRA, ALEXANDRE B. DE. Cryopreservation of banana’s cv Grand Naine in vitro rhizomes. An. Acad. Bras. Ciênc. [online]. 2018, vol.90, n.1, pp.495-507.  Epub Oct 23, 2017. ISSN 0001-3765.  https://doi.org/10.1590/0001-3765201720170056.

The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.

Keywords : Musa acumminata; in vitro conservation; cryoprotectants; pre-cooling.

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