SciELO - Scientific Electronic Library Online

 
vol.91 issue2Mapping Study on the Use of Digital Games for Persons with Disabilities in Upper MembersEvaluation of Gastro-protective Activity of Myristica fragrans on Ethanol-induced Ulcer in Albino Rats author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Anais da Academia Brasileira de Ciências

Print version ISSN 0001-3765On-line version ISSN 1678-2690

Abstract

PIRES, ALANA F. et al. Inhibitory effect of Lonchocarpus araripensis lectin in rat acute models of inflammation. An. Acad. Bras. Ciênc. [online]. 2019, vol.91, n.2, e20180991.  Epub June 19, 2019. ISSN 0001-3765.  https://doi.org/10.1590/0001-3765201920180991.

Abstract: Dalbergieae tribe lectins, possessing binding affinity for galactose and mannose, present inflammatory and nociceptive effects, while those for N-acetylglucosamine are anti-inflammatory. Since the anti-inflammatory effect of the seed lectin of L. araripensis (LAL) had been already demonstrated in mice, this effect was presently evaluated in rat models of acute inflammation. LAL (0.01-1 mg/kg) was administered by intravenous (i.v.) route in male Wistar rats 30 min before paw edema induction by dextran or carrageenan, and peritonitis by carrageenan. LAL (1 mg/kg) was incubated with N-acetylglucosamine for allowing lectin-sugar interactions before injection into animals. LAL toxicity was evaluated by the parameters: body mass, organs weight, stomach macroscopy, hematological and biochemical dosage. Statistical analysis was performed by ANOVA and Bonferroni’s test (p<0.05). The paw edema induced by carrageenan (AUC: 0.96 ± 0.09) was inhibited by LAL about 39% (0-2 h) at all doses, and about 72% (3-5 h) at 0.1 and 1 mg/kg. The increase in the neutrophil migration stimulated by carrageenan was also inhibited by LAL (83%). In both models, LAL inhibitory effect was prevented by GlcNAc. The sub-chronic treatment with LAL was well tolerated by animals. LAL possesses anti-inflammatory effect via lectin domain, indicating potential modulator role in cellular inflammatory events.

Keywords : anti-inflammatory effect; Dalbergieae; lectin domain; leguminous lectin; Loncocharpus araripensis.

        · text in English     · English ( pdf )