Exudate - phenolphthalein pH test for evaluation of validity in seeds of <i>Libidibia ferrea</i>

SOUTO, PRISCILA C.; GONÇALVES, EDILMA P.; VIANA, JEANDSON S.; SILVA, JÚLIO C.A.; FERREIRA, DÉBORA T.R.G.; RALPH, LIDIANA N.

doi:10.1590/0001-3765201920180734

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Agrarian Sciences • An. Acad. Bras. Ciênc. 91 (04) • 2019 • https://doi.org/10.1590/0001-3765201920180734 copy

Exudate - phenolphthalein pH test for evaluation of validity in seeds of Libidibia ferrea

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Abstract: Seed companies are looking for promising, quick and effective alternatives to determine the physiological quality of seeds. The objective of the current work was to study the efficiency of the exudate - phenolphthalein pH test to evaluate the seeds of two lots of Libidibia ferrea (Mart. ex Tul.) L. P. Queiroz var. ferrea. The statistical design for the the exudate - phenolphthalein pH test was completely randomized with four replicates of 50 seeds in a factorial design (2 x 5), two seed lots and five soaking periods (30, 60, 90, 120, and 150 minutes), respectively, using two constant temperatures (25 and 30°C). The percentage of viability and germination of the seeds did not differ in the temperatures of 25 and 30°C and in the soaking periods by the exudate - phenolphthalein pH test. Thus, it is recommended that the test be conducted for at least 30 minutes in distilled and deionized water at the constant temperature of 25 or 30°C to evaluate the vigor of the Libidibia ferrea.

Key words
extinction; staining; periods of imbibition; quick tests

	LOT
	1	2	CV (%)
Water content (%)	9.68a	8.60b	3.43
Weight of one tdousand seeds (g)	127.00b	144.54a	4.31
First germination count (%)	7.00b	26.00a	25.75
Germination (%)	94.00a	95.00a	3.21
Germination speed index	6.030a	6.360a	4.10
Root lengtd (cm)	7.11a	8.34a	9.41
Shoot lengtd (cm)	9.77b	10.92a	1.83
Root dry mass (g)	0.0100a	0.0100a	0.00
Shoot dry mass (g)	0.0200a	0.0200a	0.00
Cotyledons dry mass (g)	0.0100a	0.0100a	39.28

	Viab(%)	GpH (%)	WTS (g)	WC(%)	FGC(%)	GSI	G(%)	RL(cm)	SL(cm)	RDM(g)	SDM(g)	CDM(g)
Viab	-	-0.16^ns	-0.07^ns	0.54^ns	-0.57^ns	-0.36^ns	-0.16^ns	-0.61^ns	-0.57^ns	0.00^ns	0.00^ns	0.45^ns
GpH		-	-0.17^ns	-0.64^*	0.40^ns	0.16^ns	0.13^ns	0.48^ns	0.67^*	0.00^ns	0.00^ns	-0.23^ns
WTS			-	0.57^ns	-0.54^ns	-0.02^ns	0.24^ns	-0.56^ns	-0.57^ns	0.00^ns	0.00^ns	-0.06^ns
WC				-	-0.85^**	-0.43^ns	-0.22^ns	-0.53^ns	-0.90^**	0.00^ns	0.00^ns	0.31^ns
FGC					-	0.49^ns	0.18^ns	0.65^*	0.92^**	0.00^ns	0.00^ns	-0.13^ns
GSI						-	0.87^**	0.54^ns	0.60^ns	0.00^ns	0.00^ns	-0.71^*
G							-	0.33^ns	0.36^ns	0.00^ns	0.00^ns	-0.83^**
RL								-	0.79^**	0.00^ns	0.00^ns	-0.52^ns
SL									-	0.00^ns	0.00^ns	-0.38^ns
RDM										-	0.00^**	0.00^ns
SDM											-	0.00^ns
CDM												-

	Viab(%)	GpH (%)	WTS(g)	WC(%)	FGC(%)	GSI	G(%)	RL(cm)	SL(cm)	RDM(g)	SDM(g)	CDM(g)
Viab	-	0.16^ns	0.11^ns	-0.13^ns	0.11^ns	-0.63^*	-0.59^ns	0.11^ns	0.02^ns	0.00^ns	0.00^ns	0.21^ns
GpH		-	-0.31^ns	0.03^ns	0.28^ns	0.15^ns	0.26^ns	0.36^ns	0.20^ns	0.00^ns	0.00^ns	-0.15^ns
WTS			-	0.57^ns	-0.54^ns	-0.02^ns	0.24^ns	-0.56^ns	-0.57^ns	0.00^ns	0.00^ns	-0.06^ns
WC				-	-0.85^**	-0.43^ns	-0.22^ns	-0.53^ns	-0.90^**	0.00^ns	0.00^ns	0.31^ns
FGC					-	0.49^ns	0.18^ns	0.65^*	0.92^**	0.00^ns	0.00^ns	-0.13^ns
GSI						-	0.87^**	0.54^ns	0.60^ns	0.00^ns	0.00^ns	-0.71^*
G							-	0.33^ns	0.36^ns	0.00^ns	0.00^ns	-0.83^**
RL								-	0.79^**	0.00^ns	0.00^ns	-0.52^ns
SL									-	0.00^ns	0.00^ns	-0.38^ns
RDM										-	0.00^**	0.00^ns
SDM											-	0.00^ns
CDM												-

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[1] Correspondence to: Débora Teresa da Rocha Gomes Ferreira E-mail: debora_teresa@hotmail.com ORCid: https://orcid.org/0000-0001-8644-0274