Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Abstract

Oil Tea (Camellia oleifera) is an important woody edible oil plant in China. Oil Tea suffers from low rate of fruit set during production, which is related to poor pollination and fertilization. Pollen vigor is directly related to pollination and fertilization. Using the interspecific hybrid Y3 (C. grijsii × C. oleifera) as plant material, we studied the effects of sucrose, H₃BO₃, MgSO₄, and IAA on pollen germination using an orthogonal design to determine the best culture medium. Results indicated that pollen germination rates were significantly affected by medium components and ranged from 29.13% to 56.84%. Pollen tube length was the longest in the T5 medium surpassing the control group by 489.36 μm. MgSO₄ turned out to be the most important germination medium component having great effect on the pollen germination rate. The optimal culture medium to promote pollen tube growth of Oil Tea Y3 was: 1% agar, 150 g·L^-1 sucrose, 0.15 g·L^-1 H₃BO₃, 0.07 g·L^-1 MgSO₄, and 0.01 g·L^-1 IAA. The results of this paper may provide information for foliar application of Mg and IAA, which can improve pollen tube growth of Oil Tea in practice.

Key words
Oil Tea; pollen germination rate; pollen tube growth; Mg; IAA