Arquivos Brasileiros de Oftalmologia
versão impressa ISSN 0004-2749
COUTINHO, Anamaria Baptista et al. Cytokeratin expression in corneal dystrophies. Arq. Bras. Oftalmol. [online]. 2011, vol.74, n.2, pp. 118-122. ISSN 0004-2749. http://dx.doi.org/10.1590/S0004-27492011000200010.
PURPOSE: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. METHODS: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on pa- raffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). RESULTS: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. CONCLUSION: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.
Palavras-chave : Corneal dystrophies, hereditary; Keratins [physiology]; Immunohistochemistry; Cornea [pathology].