Arquivos de Neuro-Psiquiatria
On-line version ISSN 1678-4227
ARAUJO, Eduardo José de Almeida; SANT'ANA, Débora de Mello Gonçales; MOLINARI, Sônia Lucy and MIRANDA NETO, Marcílio Hubner de. Regional differences in the number and type of myenteric neurons in the descending colon of rats. Arq. Neuro-Psiquiatr. [online]. 2003, vol.61, n.2A, pp. 220-225. ISSN 1678-4227. http://dx.doi.org/10.1590/S0004-282X2003000200011.
The purpose of this study was to analyze the neuronal density of the myenteric plexus of the intermediate and antimesocolic regions of the descending colon of rats. Whole-mounts were stained with three different techniques of neuronal evidenciation. Through counts of the number of neurons in an area of 6.64 mm2 under light microscopy, we found 1,271 ± 227.54 neurons with Giemsa in the intermediate region and 1,234 ± 225.92 neurons in the antimesocolic region; with the NADH-diaphorase technique we found 530 ± 92.97 neurons in the intermediate region and 539 ± 146.72 neurons in the antimesocolic region; and through the NADPH-diaphorase histochemistry, we found 417 ± 34.42 neurons in the intermediate region and 547 ± 84.01 neurons in the antimesocolic region. We conclude that there is a variation in the density of NADPH-diaphorase positive neurons in the intestinal circumference; that the NADH-diaphorase positive neuronal subpopulation represented 42.7% of that stained with Giemsa; and that the NADPH-diaphorase positive neurons represented 37.8% of the whole myenteric population.
Keywords : myenteric plexus; Giemsa; NADH-diaphorase; NADPH-diaphorase; rat; descending colon.