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Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282X

Abstract

MICHAILOWSKY, Custódio et al. Experimental tumors of the central nervous system: standardisation of a model in rats using the 9L glioma cells. Arq. Neuro-Psiquiatr. [online]. 2003, vol.61, n.2A, pp. 234-240. ISSN 0004-282X.  http://dx.doi.org/10.1590/S0004-282X2003000200013.

A number of experimental models have been established during the last decades in order to study tumor biology and the effects of treatment or manipulation of the microenvironment of malignant glial tumors. Even though those models have been well characterised and are, to a certain extent, easily reproducible, there are limitations as to their use and to the interpretation of the results. The aim of this study is to standardize a model of a malignant glial tumor and detect possible events able to modify its development. 9L cells were inoculated intracerebrally in 48 Sprague-Dawley rats; from these, 25 animals were also implanted with a device containing electrodes for the registration of the electroencephalogramm. Animals were daily evaluated by neurologic examination. Twenty four animals developed tumor - 10 animals died either in the immediate pos-operatory period or during evolution; 14 animals did not develop tumor. Macroscopically the tumor was well demarcated from the adjacent brain; by light microscopy the tumor exhibited malignant characteristics as well as extensive infiltration of the brain parenchyma. Diagnosis was that of a malignant astrocytoma. The use of the stereotaxic frame and care to infuse a small volume of liquid containing cells during a period of 120 seconds were the most important procedures to obtain sucess in the model. Additional care should be taken in counting cells in the Neubauer camera and in maintaining cells in constant agitation before injecting the tumor-containing solution. The model here developed was efficient besides being of low cost and of relatively easy execution.

Keywords : 9L glioma; experimental brain neoplasm; Sprague-Dawley rats; inoculation technique of experimental tumors; brain tumors.

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