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Arquivos de Neuro-Psiquiatria
Print version ISSN 0004-282XOn-line version ISSN 1678-4227
MENESES, Murilo Sousa de; MONTANO PEDROSO, Juan Carlos; FUZZA, Rúbia Fátima and MILANO, Jerônimo Buzetti. Comparative analysis of human brain slices with three different staining techniques. Arq. Neuro-Psiquiatr. [online]. 2004, vol.62, n.2a, pp.276-281. ISSN 0004-282X. http://dx.doi.org/10.1590/S0004-282X2004000200016.
Studing neuroanatomy at brain slices with gray matter staining techniques has several advantages. More often, the models described by Barnard, Robert and Brown, Mulligan, and Green are used. The aim of this study was to identify which of them achieves the best results on differentiation between the gray and the white matter. Thirty coronal slices of human brains underwent staining by the three techniques, and thus compared and analysed according this three parameters: degree of differentiation between white and gray matter, presence of a single and uninterrupted line dividing the white matter from the brain cortex; and degree of impregnation of the color staining in the white matter; scores from 0 to 3 have been given for the three parameters, with total score from 0 to 9. After statistic analysis, the Barnard, Robert and Brown model showed the best results, followed by Green's and Mulligan's.
Keywords : staining methods; gray matter; neuroanatomy.