ABSTRACT
Some outer floral organs are unique in gramineous plants, like the sterile lemma and rudimentary glume in rice. However, their development mechanisms are still poorly understood. In this study, we used 4 mutants with long sterile lemma (LSL), named JF11, JF12, JF13 and JNY-7, to be crossed with Aijiaonante (AJNT) and Nipponbare (NIP), respectively. Genetic analysis indicated that LSL trait exhibited recessive heredity and was controlled by a common allele named sl-1(t). Using the method of bulk segregant analysis and linkage analysis between SSR markers and LSL trait based on F2 populations, the sl-1(t) gene was located at the interval between RM20903 and RM20948 on chromosome 7. The interval harbors a known G1 gene, which regulates the sterile lemma trait. The findings of allelic sequencing showed an 11-base deletion in gene G1 happened in the mutants of JF11, JF12 and JF13, which led to a frame-shifting mutation, whereas the mutant of JNY-7 had a base substitution that caused the change of the amino acid residue. Eight substitutions in the ORF and 10 in the upstream region from −1 to −824 were found between Indica and Japonica rice by DNA sequence analysis, but those polymorphisms have no effect on the gene function. In conclusion, we fine mapped the LSL gene, sl-1(t), and found 2 kinds of mutant alleles conferring the gene function and the DNA polymorphisms of G1 between Indica and Japonica rice.
Key words
Oryza sativa L.; gene mapping; ALOG domain
