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Jornal de Pediatria
Print version ISSN 0021-7557
MORILLO, Simone G. et al. Characterization of rotavirus strains from day care centers: pre- and post-rotavirus vaccine era. J. Pediatr. (Rio J.) [online]. 2010, vol.86, n.2, pp.155-158. ISSN 0021-7557. http://dx.doi.org/10.2223/JPED.1981.
OBJECTIVES: In 2006 the rotavirus vaccine was included in the Brazilian Immunization Program. The aim of this study was to report the results of a 5-year surveillance study of rotavirus strains in children < 5 years with acute gastroenteritis from day care centers in the state of São Paulo, Brazil. METHODS: This retrospective study was conducted with 30 day care centers from 2004 to 2008 with convenient surveillance fecal specimens, investigated by ELISA, SDS-PAGE, RT-PCR and gene sequencing to genotype characterization. RESULTS: Rotavirus infection was detected in 28.3% of samples (38/134). The most frequent genotypes detected were G9P and G1P in 2004; G1P in 2005; GNTP[NT] in 2006; G2P in 2007; and there were no cases in 2008. Mixed infections were not observed. Detection rate declined from 65.7% (23/35) in 2004 to 50% (9/18) in 2007. CONCLUSIONS: Genotype distribution varied according to collection year, accompanied by a reduction in detection rate. Use of rotavirus vaccine requires implementation of post-marketing surveillance to monitor rotavirus strain diversity and its efficacy against possible new emerging genotypes.
Keywords : Rotavirus; day care centers; gastroenteritis.