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Jornal de Pediatria
Print version ISSN 0021-7557
CILLI, Audrey et al. Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009). J. Pediatr. (Rio J.) [online]. 2011, vol.87, n.5, pp.445-449. ISSN 0021-7557. http://dx.doi.org/10.2223/JPED.2122.
OBJECTIVE: To monitor rotavirus (RV) and norovirus (NoV) infections in hospitalized children < 5 years with acute gastroenteritis in the state of São Paulo, Brazil, during a 6-year period (2004- 2009). METHODS: This retrospective study was conducted with 61 medical centers with convenient surveillance fecal specimens, investigated by enzyme-linked immunosorbent assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis, reverse polymerase chain reaction and sequencing to genotype characterization. RESULTS: RV and NoV infections were detected in 29.6% (144∕487) and 29.2% (26/89) of the samples, respectively. The most frequent RV genotypes detected were G9P in 2004; G1P in 2005; G9P in 2006; and G2P during 2007, 2008, and 2009. Detection rate declined from 36.3% (33∕91) in 2004 to 4.2% (4/95) in 2009. NoV genogroup GII was found in 61.6% (16/26) of the samples, and GI in 11.5% (3/26). Mixed NoV-RV infections were observed in 2.2% (2/89) of the samples, involving GI+G9P and GI+G2P strains. CONCLUSIONS: Genotype distribution varied according to collection year, accompanied by a reduction in detection rate. Use of RV vaccine requires implementation of post-marketing surveillance to monitor RV strain diversity and its efficacy against possible new emerging genotypes. NoVs have been increasingly identified as relevant etiological agents among hospitalized children and play an important role in the viral etiology of pediatric acute gastroenteritis in the state of São Paulo.
Keywords : Norovirus; rotavirus; children; gastroenteritis; Brazil.