Revista Brasileira de Otorrinolaringologia
versão impressa ISSN 0034-7299
XIMENES FILHO, João Aragão et al. Langerhans cells in human vocal folds mucosa: immunohistochemical study. Rev. Bras. Otorrinolaringol. [online]. 2004, vol.70, n.5, pp.584-588. ISSN 0034-7299. http://dx.doi.org/10.1590/S0034-72992004000500002.
Langerhans Cells (LC) are a type of dendritic cells that have functions which involves antigen presentation and the stimulation of a T cell response. They represent about 4% of the laryngeal epithelial cells. AIM: The aim of this study was to identify the presence of LC in the epithelium of the vocal folds, to compare their subpopulations, as well as to compare the capacity of four immunohistochemistry markers. STUDY DESIGN: Experimental. MATERIAL AND METHOD: Six cadavers, 3 men and 3 women, were studied. Analysis of vocal folds and skin paraffin blocks stained with commercially available polyclonal S-100, vimentin, CD-68 and fascin were done. After histological analysis, Student t test and Analysis of Variance (ANOVA) were accomplished in the statistical study. RESULTS AND CONCLUSIONS: It was possible to identify the presence of LC in the epithelium of the human vocal folds in no smokers of both sexes. Fascin, vimentin and CD-68 were superior markers of LC than S-100 polyclonal antibody in vocal folds (p=0,01) and in the skin (p=0,02). It was also possible to identify three different subpopulations of LC in the vocal folds and skin. However, just in the skin it was observed larger amount in the basal layer of the epithelium statistically significant.
Palavras-chave : vocal cords [anatomy & histology]; langerhans cells; immunohistochemistry.