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Revista Ceres

Print version ISSN 0034-737X


VAL, Aurinete Daienn Borges do; MOTOIKE, Sérgio Yoshimitsu; ALVARENGA, Eveline Mantovani  and  CECON, Paulo Roberto. Breaking the dormancy of niagara rosada seeds without stratification. Rev. Ceres [online]. 2010, vol.57, n.2, pp.234-238. ISSN 0034-737X.

The objective of this study was to asses the effect of three factors on the germination of grape seeds, cv. Niagara Rosada. The three studied factors were: physical treatments, chemical treatments and germination environment. The physical treatments consisted of whole seeds, seeds with a cross cut in the median region and seeds with a cut in the micropyle. The chemical treatments were applied by immersing whole seeds in aqueous solution of gibberellic acid (GA3) with concentrations of 0 and 4000 mg L-1. The environments evaluated were in vitro and ex vitro. The experiment was arranged in a factorial 3 x 2x 2, randomized blocks design, with 4 repetitions, each one consisting of a Gerbox® containing 25 seeds or a rack with 25 test tubes. The parameters evaluated were the percentage and speed of seed germination. The results showed that cutting is essential to the germination of non-stratified seeds. The combination micropyle cut, 4000 mg L-1 of GA3 and in vitro environment resulted in 77% of seed germination. The in vitro environment was significantly superior to the ex vitro environment.

Keywords : Vitis labruscana L.H. Bailey; germination; in vitro; gibberellins.

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