Test |
Objective |
Method |
Equipment |
Reference value |
|
Blood glucose |
Determination of diabetes |
Hexokinase method (enzymatic) 25
|
ADVIA 1200 Siemens® |
Fasting: 70 to 99 mg/dL 120 minutes after ingestion of glucose solution: |
< 140 mg/dL: normal tolerance to glucose 140 to 199 mg/dL: glucose intolerance |
≥ 200 mg/dL: diabetes |
Total cholesterol |
Lipid metabolism |
Cholesterol oxidase method (enzymatic colorimetric) 1
|
ADVIA 1200 Siemens® |
Desirable: < 200 mg/dL Borderline: 200-239 mg/dL High: > 240 mg/dL |
HDL-cholesterol |
Lipid metabolism |
Homogeneous, colorimetric, without precipitation 34
|
ADVIA 1200 Siemens® |
Desirable values: Non-diabetics:> 40 mg/dL diabetics: > 45 mg/dL |
Triglycerides |
Lipid metabolism |
Glycerol-phosphate peroxidase method according to Trinder (enzymatic colorimetric) 17
|
ADVIA 1200 Siemens® |
< 150 mg/dl |
LDL-cholesterol Used when triglycerides ≤ 400 mg/dl. |
Lipid metabolism |
Friedewald Equation |
|
Desirable for: |
High risk patients: < 100 mg/dL |
Medium risk patients: <130 mg/dL |
Low risk patients: < 160 mg/dL |
LDL-cholesterol Used when triglycerides > 400 mg/dl |
Lipid metabolism |
Homogeneous, enzymatic, colorimetric method without precipitation 26
|
ADVIA 1200 Siemens® |
Desirable for: |
High risk individuals: < 100 mg/dL Medium risk individuals: |
<130 mg/dL Low risk individuals: |
< 160 mg/dL |
Creatinine |
Renal function |
Jaffe’s Method 22
|
ADVIA 1200 Siemens® |
Serum: 0.4 to 1.3 mg/dL 12-hour urine: not established |
Uric acid |
Purine metabolism marker |
Uricase method (enzymatic colorimetric) 16
|
ADVIA 1200 Siemens® |
Men: 3.5 to 7.2 mg/dL |
Women: 2.6 to 6.0 mg/dL |
Aspartate aminotransferase |
Identifier for hepatic steatosis |
Modified IFCC (enzymatic) 29
|
ADVIA 1200 Siemens® |
Men: 10 to 35 U/L |
Women: 10 to 31 U/L |
Alanine |
Identificador para esteatose hepática |
Modified IFCC (enzymatic) 28
|
ADVIA 1200 Siemens® |
Men: 9 to 43 U/L |
Women: 9 to 36 U/L |
γ-glutamyl-transferase |
Identifier for hepatic and alcoholic steatosis |
Szasz Persijn (kinetic colorimetric) 30
|
ADVIA 1200 Siemens® |
Men: 2 a 30 U/L |
Women: 1 a 24 U/L |
Ultrasensitive C-Reactive Protein |
Inflammatory marker |
Immunochemistry through nephelometry 14
|
nephelometer BN II Siemens® |
For cardiovascular risk assessment: |
Low risk: < 1.0mg/L |
Medium risk: 1.0 a 3.0 mg/L |
High risk: > 3.0 mg/L |
Glycated hemoglobin |
Determination of diabetes |
High-pressure chromatography (HPLC) 20
|
Variant Bio Rad® |
< 5.7% Normal tolerance to glucose |
TSH |
Etiological investigation |
Immunoenzymatic assay 23,35
|
Centaur Siemmens® |
0.55 – 4.78 mcUI/mL |
Free T4 |
Etiological investigation |
Immunoenzymatic assay 26,35
|
Centaur Siemmens® |
0.89 to 1.76 ng/dL |
Insulin |
Carbohydrate metabolism |
Immunoenzymatic assay 23,35
|
Centaur Siemmens® |
Fasting:3.0 - 25.0 mUI/L After ingestion of glucose solution: |
Not established |
Urine Na |
Food intake |
Potentiometry with ion-selective electrode |
ADVIA 1200 Siemens® |
Not established |
Urine K |
Food intake |
Potentiometry with ion-selective electrode |
ADVIA 1200 Siemens® |
Not established |
Urine calcium |
Food intake |
Potentiometry with ion-selective electrode) 18
|
ADVIA 1200 Siemens® |
Not established |
Microalbuminuria |
Endothelial function |
Immunochemistry through nephelometry |
nephelometer BN II Siemens® |
Normal: < 20 μg/m in |
Microalbuminuria: 20 to 200 μg/min |
Macroalbuminuria: > 200 μg/min |
Serology for Chagas Disease |
Differential diagnosis in cardiomyopathies |
ELISA with solid-phase microplates – reagent WIENER (CHAGASTEST) 15
|
Microplate incubator/reader |
Not reactive |