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Revista do Instituto de Medicina Tropical de São Paulo
On-line version ISSN 1678-9946
CORREA, Benedito et al. Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor. Rev. Inst. Med. trop. S. Paulo [online]. 1990, vol.32, n.1, pp. 46-50. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46651990000100008.
The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells.
Keywords : Cryptococcus neoformans; Viabilidade; Fluorescência.