SciELO - Scientific Electronic Library Online

vol.32 issue1The prevalence of antibody to human parvovirus B19 in Rio de Janeiro, BrazilAcute toxoplasmosis: evaluation of the thin-layer immunoassay for detecting IgM antibodies to Toxoplasma gondii author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946


CORREA, Benedito et al. Fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor. Rev. Inst. Med. trop. S. Paulo [online]. 1990, vol.32, n.1, pp.46-50. ISSN 1678-9946.

The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells.

Keywords : Cryptococcus neoformans; Viabilidade; Fluorescência.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License