Revista do Instituto de Medicina Tropical de São Paulo
versão impressa ISSN 0036-4665
FIGUEREDO-SILVA, José et al. Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods. Rev. Inst. Med. trop. S. Paulo [online]. 1991, vol.33, n.3, pp. 193-198. ISSN 0036-4665. http://dx.doi.org/10.1590/S0036-46651991000300005.
A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.
Palavras-chave : Trypanosoma cruzi; Epidemiological survey; Serodiagnosis; Indirect immunofluorescence; Enzyme-linked immunosorbent assay; Competitive enzyme-linked immunosorbent assay.