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Revista do Instituto de Medicina Tropical de São Paulo
On-line version ISSN 1678-9946
SOUZA, Hertha Barbara Wüllert Telles de et al. Attempts for breeding triatominae in laboratory by means of "in vitro" feeding. Rev. Inst. Med. trop. S. Paulo [online]. 1991, vol.33, n.3, pp. 221-226. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46651991000300010.
In order to simplify breeding of triatominae in the laboratory, for performing xenodiagnosis and other biologic studies, we tried to feed the insects "in vitro" with citrated or defibrinated blood from commercially abated chicken. Two types of efficacy observations were carried out with Triatoma infestans: a) analysis of the chaining of successive nymphal stages, viability of satisfactory matching, fertile oviposition and adequate reproduction; b) assessment of viability and infectivity of Trypanosoma cruzi in the insects. As a conclusion, it became evidente that, despite operational easiness, the objectives were not achieved, since the classical procedure used as the control, was always superior.
Keywords : Criação de triatomíneos; Alimentação "in vitro"; Desenvolvimento; Infecção pelo Trypanosoma cruzi.