Revista do Instituto de Medicina Tropical de São Paulo
On-line version ISSN 1678-9946
TIRABOSCHI, Iris Nora; MARTICORENA, B. and NEGRONI, R.. E.L.I.S.A. in human coccidioidomycosis . Rev. Inst. Med. trop. S. Paulo [online]. 1991, vol.33, n.4, pp.281-285. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46651991000400007.
An E.L.I.S.A. test for antibody detection, with an exo-antigen of Coccidioides immitis was standardized in 67 humans sera diluited in 1/1000, 1/2000, 1/4000 and 1/8000. Eightheen sera from mycologically proved cases of coccidioidomycosis were studied: 5 were negative and 13 were positive in some dilutions. 3/26 sera of healthy persons who presented positive skin tests with coccidioidin were positive and the other 23 sera did not have positive reactions. None of the 15 sera of healthy human exhibited positive E.L.I.S.A. Serum samples of 8 patients suffering other deep mycosis were studied, 4 of them presented cross-reactions in E.L.I.S.A. tests. E.L.I.S.A. test seems to be a useful Serologic technique for antibody detection in anticomplementary serum samples or when a low concentration of antibodies should be detected. As it is very sensitive, cross-reactions with other mycoses are frequent, thus the use other more specific serologic technique together E.L.I.S.A. is recommended.
Keywords : Coccidioidomicosis; E.L.I.S.A.; Coccidioides immitis.