SciELO - Scientific Electronic Library Online

 
vol.33 issue4Evaluation of the basic procedures involved in the storage of measles vaccine in "Public Health Units" of the Municipalities of Niterói and São Gonçalo, State of Rio de Janeiro, BrazilProposal of a new process for screening of drugs in experimental infection with Trypanosoma cruzi author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Revista do Instituto de Medicina Tropical de São Paulo

Print version ISSN 0036-4665

Abstract

CANO, Maria Isabel Nogueira  and  AGUIAR, Mônica S.M. Vidal de. The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism. Rev. Inst. Med. trop. S. Paulo [online]. 1991, vol.33, n.4, pp. 319-324. ISSN 0036-4665.  http://dx.doi.org/10.1590/S0036-46651991000400013.

Fiftenn Paracoccidioides brasiliensis strains, in the mycelial (M) and yeast like (Y), were cultivated in minimal medium (MM) and subcultivated to be adapted to the same medium supplemented with a pool of amminoacid in solution (MMS). Each of the aminoacids were studied separately of the solution to provide the auxological study. The prototrophism was demonstrated by nine strains in both M and Y forms, and the auxotrofism by the remaining strains. The heterologous results has not allowed us to draw an auxological characterization of the P. brasiliensis. As far as we could observe none of the aminoacid studied in this piece of research can be considered of absolute importance for to the growth and the morphogenesis maintainance of the fungus. Morphological alterations were only verified in the prototroph strains, which suggest that there could have been adaptative metabolism activity due to the absence of organic nitrogen compounds in the minimal medium (MM).

Keywords : Paracoccidioides brasiliensis; Aminoácidos; Dimorfismo.

        · abstract in Portuguese     · text in Portuguese     · pdf in Portuguese