Revista do Instituto de Medicina Tropical de São Paulo
Print version ISSN 0036-4665
ELSAID, Mohamed Mohamed Anwar; BAHIA, Maria Terezinha; MACHADO-COELHO, George Luiz and VITOR, Ricardo Wagner de Almeida. Diagnosis of human toxoplasmosis by a dot enzyme-linked immunosorbent assay. Rev. Inst. Med. trop. S. Paulo [online]. 1995, vol.37, n.2, pp. 117-122. ISSN 0036-4665. http://dx.doi.org/10.1590/S0036-46651995000200005.
A Dot enzyme-linked immunosorbent assay (Dot-ELISA) was standardized and evaluated for the serodiagnosis of human toxoplasmosis. Out of 538 serum samples tested by the immunofluorescence test for toxoplasmosis (IFAT-IgG) as reference test, 183 (34%) were positive at cut off 1:16 and 192 (36%) were positive for Dot-ELISA-IgG at cut-off 1:256. For Dot-ELISA, co-positivity was 0.94, co-negativity 0.94 and concordance 0.88 in relation to IFAT-IgG. These results suggest the usefulness of Dot-ELISA (cut-off titer of 1:256) for the serodiagnosis of human toxoplasmosis. The main advantage of this technique is simplicity, positive test can be visually identified (colored precipitate). It does not require a special equipment and it can be used as a qualitative test to screen large numbers of samples or as a quantitative assay to determine end-point titration of individual sera.
Keywords : Human toxoplasmosis; Dot-ELISA; Serodiagnosis.