A simple PCR-based procedure for plaque diagnosis

Um método simples para o diagnóstico de peste por PCR

Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.

Plague; Yersinia pestis; Rodents; PCR; Diagnosis