SciELO - Scientific Electronic Library Online

 
vol.39 issue4QUANTIFICATION OF THE POPULATION AND PHAGOCYTARY ACTIVITY OF HEMOCYTES OF RESISTANT AND SUSCEPTIBLE STRAINS OF Biomphalaria glabrata AND Biomphalaria tenagophila INFECTED WITH Schistosoma mansoniPeptic disease and Helicobacter pylori are highly prevalent in patients with the indeterminate form of chagas’ disease: report of 21 cases author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Revista do Instituto de Medicina Tropical de São Paulo

Print version ISSN 0036-4665

Abstract

PARMA, Alberto E. et al. Differentiation of pathogenic and non-pathogenic leptospires by means of the polymerase chain reaction. Rev. Inst. Med. trop. S. Paulo [online]. 1997, vol.39, n.4, pp. 203-208. ISSN 0036-4665.  http://dx.doi.org/10.1590/S0036-46651997000400004.

A polymerase chain reaction was carried out to detect pathogenic leptospires isolated from animals and humans in Argentina. A double set of primers (G1/G2, B64-I/B64-II), described before, were used to amplify by PCR a DNA fragment from serogroups belonging to Leptospira interrogans but did not allow to detect saprophytic strains isolated from soil and water (L. biflexa). This fact represents an advantage since it makes possible the differentiation of pathogenic from non-pathogenic leptospires in cultures. The sensitivity of this assay has been determined, allowing to detect just only 10 leptospires in the reaction tube. Those sets of primers generated either a 285 bp or 360 bp fragment, depending on the pathogenic strain

Keywords : Polymerase chain reaction; Leptospira biflexa; L. interrogans.

        · abstract in Portuguese     · text in English