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Revista do Instituto de Medicina Tropical de São Paulo

Print version ISSN 0036-4665

Abstract

VEGA, Benito; GARCIA, Jorge D.  and  HERNANDEZ, Francisco. Immunoglobulin Fc receptors in clinical strains of Staphylococcus aureus do not confer resistance to Phagocytosis in an in vitro assay. Rev. Inst. Med. trop. S. Paulo [online]. 1999, vol.41, n.3, pp. 143-145. ISSN 0036-4665.  http://dx.doi.org/10.1590/S0036-46651999000300001.

Staphylococcus aureus binds Immunoglobulin G (IgG) on its external surface due to the presence of specific receptors for the Fc domain of this immunoglobulin. This mechanism represents a kind of camouflage against phagocytic cells. In order to confirm that possibility an in vitro evaluation of the phagocytic activity of leukocytes polymorpho-nuclear (PMN) against strains of Staphylococcus aureus was done, comparing 18 strains isolated from clinical samples and 16 from healthy individuals. The presence of Fc receptors was evaluated by haemagglutination (HA) with erythrocytes group A after incubation of the strains with IgG anti blood group A. Phagocytosis of S. aureus was carried out by mixing live bacteria with a suspension of human PMN and incubating at 37 °C for 1 h; survivors were counted as colony forming units by plating. The strains from clinical specimens showed higher HA than those from healthy individuals (p = 0.01); but the former were killed more efficiently than the latter (80-90% and 40%, respectively). It is may be possible that S. aureus showed different behavior in vivo, where could express other virulence factors to prevent the action of phagocytes.

Keywords : Staphylococcus aureus; Fc receptors and phagocytosis; Phagocytic evasion; Polymorphonuclear leukocytes; Haemagglutination.

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