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Revista do Instituto de Medicina Tropical de São Paulo
On-line version ISSN 1678-9946
MALAQUE, Ceila M.S.; ORI, Maria; SANTOS, Sânia A. and ANDRADE, Dahir R.. Production of TNF-a by primary cultures of human keratinocytes challenged with Loxosceles gaucho venom. Rev. Inst. Med. trop. S. Paulo [online]. 1999, vol.41, n.3, pp. 179-182. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46651999000300009.
Primary cultures of human keratinocytes were challenged with increasing doses from 10 ng/mL to 2 mg/mL of Loxosceles gaucho venom, responsible for dermonecrotic lesion in humans. TNF-a was investigated by bioassay and ELISA in the supernatant of the cultures challenged with 100 ng/mL, 500 ng/mL, 1 and 2 mg/mL of venom. TNF-a was detected by bioassay in the supernatant of cultures challenged with 100 ng/mL, after 6 h. The cytokine was detected by ELISA in the supernatant of the cells challenged with doses of l mg/mL, after 6 and 12 h. The results point out the capacity of this venom to activate the keratinocytes in primary cultures to produce TNF-a. The production of cytokines could contribute to the local inflammatory process in patients bitten by Loxosceles sp.
Keywords : Loxosceles; Keratinocytes; Cell culture; TNF-a.