Revista do Instituto de Medicina Tropical de São Paulo
versão impressa ISSN 0036-4665
MIAGOSTOVICH, Marize P. et al. Molecular typing of dengue virus type 2 in Brazil. Rev. Inst. Med. trop. S. Paulo [online]. 2003, vol.45, n.1, pp. 17-21. ISSN 0036-4665. http://dx.doi.org/10.1590/S0036-46652003000100004.
Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).
Palavras-chave : Dengue virus type 2; Molecular epidemiology; Brazil.