Revista do Instituto de Medicina Tropical de São Paulo
Print version ISSN 0036-4665
ONO, Erika et al. A simple and cheaper in house varicella zoster virus antibody indirect ELISA. Rev. Inst. Med. trop. S. Paulo [online]. 2004, vol.46, n.3, pp. 165-168. ISSN 0036-4665. http://dx.doi.org/10.1590/S0036-46652004000300008.
We have developed a cheaper an simple in house indirect ELISA that uses the live attenuated VZV vaccine as a coating antigen. The alternative ELISA had an agreement of 94% when compared with a commercial VZV ELISA kit. Moreover, our ELISA proved to be more reliable than the kit when assessing true negative samples. By adding a standard serum, we were able to produce results in international units per millilitre. Also, the addition of an extra step with 8M urea allowed the assessment of VZV IgG avidity without excessive costs. The cost per sample to test VZV IgG was 2.7 times cheaper with our ELISA, allowing the testing of many samples without the burden of production of VZV antigen in the laboratory.
Keywords : Varicella; ELISA; Antibody avidity.