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Revista do Instituto de Medicina Tropical de São Paulo

Print version ISSN 0036-4665

Abstract

BERNARDO, Wagner Luis de Carvalho; BORIOLLO, Marcelo Fabiano Gomes; GONCALVES, Reginaldo Bruno  and  HOFLING, José Francisco. Staphylococcus aureus ampicillin-resistant from the odontological clinic environment. Rev. Inst. Med. trop. S. Paulo [online]. 2005, vol.47, n.1, pp. 19-24. ISSN 0036-4665.  http://dx.doi.org/10.1590/S0036-46652005000100004.

The aim of this research was to evaluate the prevalence of Sthaphylococcus spp. and S. aureus in the odontological clinic environment (air), their production of b-lactamase and antibacterial susceptibility to the major antibiotics utilized in medical particle. During 12 months of samples collect were isolated 9775 CFU by MSA medium suggesting a high amount of Staphylococcus spp. in the clinic environment which can appear through aerosols. A total of 3149 colonies (32.2%) were suggestive of pathogenic staphylococci. Gram coloration, catalase test, colony-mallow growing on chromogenic medium, and coagulase test confirmed the identity of 44 (0.45%) S. aureus isolates. Of these, 35 isolates (79.5%) showed production of b-lactamase by CefinaseTM discs and resistance to ampicillin, erythromycin (7 isolates) and tetracycline (1 isolate) suggesting the existence of multiresistant isolates. The evaluation of the oxacillin MIC by Etest® assays showed susceptibility patterns suggesting the inexistence of the mecA gene in chromosomal DNA. These results point out to the need of a larger knowledge on the contamination means and propagation of this microorganism into the odontological clinic.

Keywords : Staphylococcus aureus; Ampicillin-resistant; Odontological clinic environment.

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