Abstract

AVILA-CAMPOS, Mario J.; RIVERA, Irma N.  and  NAKANO, Viviane. Genetic diversity of oral Fusobacterium nucleatum isolated from patients with different clinical conditions. Rev. Inst. Med. trop. S. Paulo [online]. 2006, vol.48, n.2, pp. 59-63. ISSN 0036-4665.  http://dx.doi.org/10.1590/S0036-46652006000200001.

The genetic diversity of 23 oral Fusobacterium nucleatum isolated from 15 periodontal patients, eight from seven healthy subjects, nine from nine AIDS patients and two from two Cebus apella monkeys were analyzed. EcoRI restricted the bacterial DNA and 28 ribotypes grouped from A to J groups were obtained. Isolates formed 24 ribotypes which were contained into A, B, C, D, E and F groups, and three reference strains and two clinical isolates of A. actinomycetemcomitans, and E. coli CDC formed four different ribotypes into the G, H, I and J groups. Moreover, from nine F. nucleatum from AIDS patients, six were ribotyped as group C and three as group D. By using ribotyping we distinguished F. nucleatum recovered from different sources. It is possible that isolates from AIDS patients may contain some phenotypic or genotypic factor did not observed in this study.

Keywords : Anaerobic bacteria; Periodontopathogens; Periodontal disease; Fusobacterium nucleatum; Ribotyping.