Revista do Instituto de Medicina Tropical de São Paulo
On-line version ISSN 1678-9946
SOUZA, Thais Furtado de et al. Secretion of five extracellular enzymes by strains of chromoblastomycosis agents. Rev. Inst. Med. trop. S. Paulo [online]. 2008, vol.50, n.5, pp.269-272. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46652008000500004.
The gelatinase, urease, lipase, phospholipase and DNase activities of 11 chromoblastomycosis agents constituted by strains of Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala jeanselmei were analyzed and compared. All strains presented urease, gelatinase and lipase activity. Phospholipase activity was detected only on five of six strains of F. pedrosoi. DNase activity was not detected on the strains studied. Our results indicate that only phospholipase production, induced by egg yolk substrate, was useful for the differentiation of the taxonomically related species studied, based on their enzymatic profile.
Keywords : Chromoblastomycosis; Fonsecaea pedrosoi; Lipase; Phospholipase; DNAse; Urease; Gelatinase.