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Revista do Instituto de Medicina Tropical de São Paulo
On-line version ISSN 1678-9946
VIANNA, Manoella Campostrini Barreto et al. Rickettsial spotted fever in capoeirão Village, Itabira, Minas Gerais, Brazil. Rev. Inst. Med. trop. S. Paulo [online]. 2008, vol.50, n.5, pp.297-301. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46652008000500009.
The present study investigated the infection by spotted fever rickettsia in an endemic area for Brazilian spotted fever (BSF; caused by Rickettsia rickettsii) in Minas Gerais State, Brazil. Human, canine and equine sera samples, and Amblyomma cajennense adult ticks collected in a rural area of Itabira City, Minas Gerais State were tested for rickettsial infection. Through Immunofluorescence Assay (IFA) we demonstrated the presence of antibodies anti-R. rickettsii in 8.2%, 81.3% and 100% of the human, canine and equine sera, respectively. None of the 356 tick specimens analyzed were positive for Rickettsia by the hemolymph test or Polymerase Chain Reaction technique (PCR) for the htrA and the gltA genes. Our serological results on horses and dogs (sentinels for BSF) appoint for the circulation of a SFG Rickettsia in the study area, however in a very low infection rate among the A. cajennense tick population.
Keywords : Spotted fever; Rickettsia rickettsii; Indirect Immunofluorescence Assay; PCR; Itabira.