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Revista do Instituto de Medicina Tropical de São Paulo
On-line version ISSN 1678-9946
ARAUJO, Crystiane Rodrigues et al. In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method. Rev. Inst. Med. trop. S. Paulo [online]. 2009, vol.51, n.1, pp.9-12. ISSN 1678-9946. http://dx.doi.org/10.1590/S0036-46652009000100002.
The antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from Goiania city patients, Brazil. In this study, the microtiter plates were incubated at 28 ºC allowing a reading of the minimal inhibitory concentration (MIC) after four days of incubation for Trichophyton mentagrophytes and five days for T. rubrum and Microsporum canis. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 0.03 µg/mL were found for 33.3%, 31.6% and 15% of isolates for itraconazole, ketoconazole and terbinafine, respectively.
Keywords : Dermatophytes; Broth microdilution method; Minimal Inhibitory Concentration.