Revista do Instituto de Medicina Tropical de São Paulo
versión impresa ISSN 0036-4665
SAVANI, Elisa San Martin Mouriz et al. First occurrence of an autochthonous canine case of Leishmania (Leishmania) infantum chagasi in the municipality of Campinas, State of São Paulo, Brazil. Rev. Inst. Med. trop. S. Paulo [online]. 2011, vol.53, n.4, pp. 227-229. ISSN 0036-4665. http://dx.doi.org/10.1590/S0036-46652011000400010.
An autochthonous case of visceral leishmaniasis is reported in a dog (Canis familiaris) as an apparently natural infection in a non-endemic area. DNA obtained from spleen and liver samples produced the expected fragment in a Leishmania-specific rDNA-based nested-PCR assay. The PCR product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of Leishmania (Leishmania) infantum chagasi. These results are surprising since no autochthonous human or canine cases of visceral leishmaniasis have ever been reported in this municipality. This case suggests that natural transmission of this disease is occurring in this area.
Palabras llave : Visceral leishmaniasis; PCR; Epidemiology; Dogs; São Paulo; Brazil.