Revista da Sociedade Brasileira de Medicina Tropical
On-line version ISSN 1678-9849
EGIDO, Jesús M. and VINUELAS, Juan. Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans. Rev. Soc. Bras. Med. Trop. [online]. 1997, vol.30, n.6, pp.441-446. ISSN 1678-9849. http://dx.doi.org/10.1590/S0037-86821997000600001.
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
Keywords : Phagocytosis; Flow cytometry; Latex particles; Candida albicans.