SciELO - Scientific Electronic Library Online

 
vol.30 issue6Efetividade do Programa de Comunicação e Educação em Saúde no controle da infecção por S. mansoni em algumas áreas do Estado da Bahia author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682

Abstract

EGIDO, Jesús M.  and  VINUELAS, Juan. Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans. Rev. Soc. Bras. Med. Trop. [online]. 1997, vol.30, n.6, pp. 441-446. ISSN 0037-8682.  http://dx.doi.org/10.1590/S0037-86821997000600001.

We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.

Keywords : Phagocytosis; Flow cytometry; Latex particles; Candida albicans.

        · abstract in Spanish     · text in English     · pdf in English