Revista da Sociedade Brasileira de Medicina Tropical
versión impresa ISSN 0037-8682
versión On-line ISSN 1678-9849
MUTANI, Alex y KAMINJOLO, James Stwart. The value of in vitro cell culture of granulocytes in the detection of Ehrlichia. Rev. Soc. Bras. Med. Trop. [online]. 2001, vol.34, n.4, pp.377-380. ISSN 0037-8682. http://dx.doi.org/10.1590/S0037-86822001000400012.
Peripheral blood leukocytes from different animals were isolated from whole blood and maintained in Dulbeco's medium containing homologous serum without antibiotics. After 72 hrs microscopic examination of these cells showed that most animals were infected with Ehrlichia. Observation of thin blood smears from the same animals showed that only two were positive for Ehrlichia. The results of this investigation show that leukocyte culture is superior to the traditional thin blood film method in the detection of Ehrlichia and that asymptomatic carriers are easily detected. The method is inexpensive and does not require specific cell lines although it is necessary to use sterile sera.
Palabras clave : Ehrlichia; Cell Culture; Mononuclear cells.