Revista da Sociedade Brasileira de Medicina Tropical
versión impresa ISSN 0037-8682
versión On-line ISSN 1678-9849
BRIENZE, Vânia Maria Sabadoto et al. Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil. Rev. Soc. Bras. Med. Trop. [online]. 2001, vol.34, n.4, pp.389-393. ISSN 0037-8682. http://dx.doi.org/10.1590/S0037-86822001000400015.
Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.
Palabras clave : Diagnosis of tuberculous meningitis; PCR; Mycobacterium tuberculosis.