Revista da Sociedade Brasileira de Medicina Tropical
On-line version ISSN 1678-9849
BEZERRA, Claudia C.F. et al. Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. Rev. Soc. Bras. Med. Trop. [online]. 2006, vol.39, n.3, pp.241-244. ISSN 1678-9849. http://dx.doi.org/10.1590/S0037-86822006000300002.
Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.
Keywords : Coccidioides immitis; Long-term preservation; PCR.