SciELO - Scientific Electronic Library Online

 
vol.41 número1Serum levels of interleukin-6, tumor necrosis factor-alpha and interferon-gama in infants with and without dengue índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Revista da Sociedade Brasileira de Medicina Tropical

versión impresa ISSN 0037-8682

Resumen

ROCHA, Bruno Aragão et al. Identification and differentiation of Candida species from pediatric patients by random amplified polymorphic DNA. Rev. Soc. Bras. Med. Trop. [online]. 2008, vol.41, n.1, pp. 1-5. ISSN 0037-8682.  http://dx.doi.org/10.1590/S0037-86822008000100001.

Thirty-four Candida isolates were analyzed by random amplified polymorphic DNA using the primer OPG-10:24 Candida albicans; 4 Candida tropicalis; 2 Candida parapsilosis; 2 Candida dubliniensis; 1 Candida glabrata and 1 Candida krusei. The UPGMA-Pearson correlation coefficient was used to calculate the genetic distance between the different Candida groupings. Samples were classified as identical (correlation of 100%); highly related samples (90%); moderately related samples (80%) and unrelated samples (< 70%). The results showed that the RAPD proposed was capable of classifying the isolates coherently (such that same species were in the same dendrogram), except for two isolates of Candida parapsilosis and the positive control (Netherlands, 1973), probably because they are now recognized as three different species. Concerning the only fluconazole-resistant Candida tropicalis isolate with a genotype that was different to the others, the data were insufficient to affirm that the only difference was the sensitivity to fluconazole. We concluded that the Random Amplified Polymorphic DNA proposed might be used to confirm Candida species identified by microbiological methods.

Palabras llave : RAPD; Candidiasis; Candida spp; Polymorphism; Molecular typing.

        · resumen en Portugués     · texto en Inglés     · pdf en Inglés