Revista da Sociedade Brasileira de Medicina Tropical
Print version ISSN 0037-8682
COSTA, Maria Sandra Moura et al. Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia. Rev. Soc. Bras. Med. Trop. [online]. 2012, vol.45, n.4, pp. 520-522. ISSN 0037-8682. http://dx.doi.org/10.1590/S0037-86822012000400021.
INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P, G3P, and G4P genotypes. The G1P genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.
Keywords : Group A rotavirus; Genotyping; Brazilian Amazonia.