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Short Communication • Rev. Soc. Bras. Med. Trop. 48 (6) • Dec 2015 • https://doi.org/10.1590/0037-8682-0076-2015   copy

Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract:

INTRODUCTION

: This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil.

METHODS

: Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing.

RESULTS

: PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively.

CONCLUSIONS

: Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.

Keywords:
PCR; Trypanosomatids; Phlebotominae.

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